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Endothelial Nitric Oxide Synthase Tagging Single Nucleotide Polymorphisms and Recovery From Aneurysmal Subarachnoid Hemorrhage

School of Nursing, University of Pittsburgh Pennsylvania, salexand{at}pitt.edu

Samuel Poloyac, PhD

Pharmaceutical Sciences, University of Pittsburgh Pennsylvania

Leslie Hoffman, RN, PhD

School of Nursing, University of Pittsburgh Pennsylvania

Matthew Gallek, RN, PhD

College of Nursing, The University of Arizona, Tucson, Arizona

Dianxu Ren, PhD

School of Nursing, University of Pittsburgh Pennsylvania

Jeffrey Balzer, PhD

Neurological Surgery University of Pittsburgh Pennsylvania

Amin Kassam, MD

Neurological Surgery, University of Pittsburgh Pennsylvania

Yvette Conley, PhD

School of Nursing, University of Pittsburgh Pennsylvania

Aneurysmal subarachnoid hemorrhage (SAH) is a hemorrhagic stroke subtype with a poor recovery profile. Cerebral vasospasm (CV), a narrowing of the cerebral vasculature, significantly contributes to the poor recovery profile. Variation in the endothelial nitric oxide (NO) synthase (eNOS) gene has been implicated in CV and outcome after SAH. The purpose of this project was to explore the potential association between three eNOS tagging single nucleotide polymorphisms (SNPs) and recovery from SAH. We included 195 participants with a diagnosis of SAH and DNA and 6-month outcome data available but without preexisting neurologic disease/deficit. Genotyping was performed using an ABI Prism 7000 Sequence Detection System and TaqMan assays. CV was verified by cerebral angiogram independently read by a neurosurgeon on 118 participants. Modified Rankin Scores (MRS) and Glasgow Outcome Scale (GOS) scores were collected 6 months posthemorrhage. Data were analyzed using descriptive statistics, analysis of variance (ANOVA) and chi-square analysis as appropriate. The sample was primarily female (n = 147; 75.4%) and White (n = 178; 91.3%) with a mean age of 54.6 years. Of the participants with CV data, 56 (47.5%) developed CV within 14 days of SAH. None of the SNPs individually were associated with CV presence; however, a combination of the three variant SNPs was significantly associated with CV (p = .017). Only one SNP (rs1799983, variant allele) was associated with worse 6-month GOS scores (p < .001) and MRS (p < .001). These data indicate that the eNOS gene plays a role in the response to SAH, which may be explained by an influence on CV.

Key Words: subarachnoid hemorrhage • cerebral vasospasm • nitric oxide • genotype • outcome

This version was published on July 1, 2009

Biological Research For Nursing, Vol. 11, No. 1, 42-52 (2009)
DOI: 10.1177/1099800409334751


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