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Biological Research For Nursing, Vol. 5, No. 2, 142-152 (2003)
DOI: 10.1177/1099800403257196

Natural Killer Cell Cytotoxicity: A Methods Analysis of Versus Flow Cytometry Chromium Release

Sandra Adams Motzer, PhD, RN, FAHA

Department of Biobehavioral Nursing and Health Systems, Box 357266, University of Washington, Seattle, WA 98195-7266, underhil{at}u.washington.edu

Joyce Tsuji, BA

Biobehavioral Immune Laboratory

Vicky Hertig, PhC, RN

"NK Cell Activity and IBS Across the Menstrual Cycle"

Sandra K. Johnston, PhC, RN

"NK Cell Activity and IBS Across the Menstrual Cycle" in the Department of Biobehavioral Nursing and Health Systems, School of Nursing, University of Washington, Seattle

James Scanlan, PhD

School of Medicine, Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle

The purpose of this study is to describe design considerations for the use of flow cytometry (FC) com-pared to 51chromium (51Cr)-release assays utilizing cryopreserved peripheral blood mononuclear cells (PBMCs) to detect natural killer (NK) cell cytotoxicity. Subjects were 10 healthy women aged 18 to 39 years. Intra-assay variability between methods differed only at the lowest effector-target ratios evaluated. Interassay variability was wide but did not differ between methods. The relationship of lytic unit-10 between methods was strongly positive. Cytotoxicity detected by 51Cr release was higher than that detected by FC for all 10 subjects. Cost was comparable. How-ever, had more assays been performed, technician time would have been greater with flow cytometry. More whole blood was needed to perform the flow cytometry cytotoxicity assay than 51Cr-release cytotoxicity assay. The authors found no compelling reason to adopt NK cell cytotoxicity by flow cytometry over 51Cr release.

Key Words: natural killer cells • 51chromium-release assay • flow cytometry • cytotoxicity


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