This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Goodyear-Bruch, C. A. Articles by Pierce, J. D. Search for Related Content PubMed PubMed Citation Articles by Goodyear-Bruch, C. A. Articles by Pierce, J. D. Social Bookmarking                         What's this?

Apoptotic-Related Protein Expression in the Diaphragm and the Effect of Dopamine During Inspiratory Resistance Loading

From School of Allied Health

Jay Jegathesan, MS

School of Nursing, University of Kansas Medical Center, Kansas City, Kansas

Richard L. Clancy, PhD

School of Nursing, University of Kansas Medical Center, Kansas City, Kansas

Janet D. Pierce, DSN, ARNP, CCRN

School of Nursing, University of Kansas Medical Center, Kansas City, Kansas, jpierce{at}kumc.edu

Dopamine (DA) is a free radical scavenger that attenuates apoptosis. We studied the effects of normal saline (NS) and DA on diaphragm apoptotic protein expression following 60 min of inspiratory resistance loading in rats. We tested for 27 apoptotic-related proteins and found 12 in the diaphragm. Of the 12 proteins, superoxide dismutase copper zinc (SOD [CuZn]) and proprioceptive event related potential (PERP) were significantly higher in the DA group than in the NS and sham groups (p = .002, p = .007). DA group diaphragms had significantly greater expression of SOD (CuZn) than the NS (p = .005) and sham group diaphragms (p = .003). Likewise, the DA group had significantly greater expression of PERP than the NS group (p = .008). These results suggest that DA decreases diaphragm apoptosis through elevated expression of SOD (CuZn). The identification of 12 apoptotic-related proteins will assist investigators as they study diaphragm apoptosis.

Key Words: diaphragm • apoptosis • dopamine • proteins • free radical • rats • scavengers

References

• Callahan, L.A., Nethery, D., Stofan, D., DiMarco, A., & Supinski, G. (2001). Free radical-induced contractile protein dysfunction in endotoxin-induced sepsis. American Journal of Respiratory Cell and Molecular Biology, 24, 210-217.[Abstract/Free Full Text]
• Callahan, L.A., & Supinski, G.S. (2005). Downregulation of diaphragm electron transport chain and glycolytic enzyme gene expression in sepsis. Journal of Applied Physiology, 99, 1120-1126.[Abstract/Free Full Text]
• Chae, I.H., Park, K.W., Kim, H.S., & Oh, B.H. (2004). Nitric oxide-induced apoptosis is mediated by Bax/Bcl-2 gene expression, transition of cytochrome C, and activation of caspase-3 in rat vascular smooth muscle cells. Clinica Chimica Acta, 341, 83-91.[CrossRef][Medline] [Order article via Infotrieve]
• Chao, C., Saito, S., Kang, J., Anderson, C.W., Appella, E., & Xu, Y. (2000). p53 transcriptional activity is essential for p53-dependent apoptosis following DNA damage. EMBO Journal, 19, 4967-4975.[CrossRef][Medline] [Order article via Infotrieve]
• Childs, A.C., Phaneuf, S.L., Dirks, A.J., Phillips, T., & Leeuwenburgh, C. (2002). Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2:Bax ratio. Cancer Research, 62, 4592-4598.[Abstract/Free Full Text]
• Didion, S.P., & Faraci, F.M. (2005). Ceramide-induced impairment of endothelial function is prevented by CuZn superoxide dismutase overexpression. Arteriosclerosis, Thrombosis, and Vascular Biology, 25, 90-95.[Abstract/Free Full Text]
• Didion, S.P., Ryan, M.J., Didion, L.A., Fegan, P.E., Sigmund, C.D., & Faraci, F.M. (2002). Increased superoxide and vascular dysfunction in CuZn SOD-deficient mice. Circulation Research, 91, 938-944.[Abstract/Free Full Text]
• Falk, D.J., Deruisseau, K.C., Van Gammeren, D.L., Deering, M.A., Kavazis, A.N., & Powers, S.K. (2006). Mechanical ventilation promotes redox status alterations in the diaphragm. Journal of Applied Physiology, 101, 1017-1024.[Abstract/Free Full Text]
• Fujimura, N., Sumita, S., Aimono, M., Masuda, Y., Shichinohe, Y., Narimatsu, E., et al. (2000). Effect of free radical scavengers on diaphragmatic contractility in septic peritonitis. American Journal of Respiratory and Critical Care Medicine, 162, 2159-2165.[Abstract/Free Full Text]
• Gosker, H.R., Wouters, E.F., van der Vusse, G.J., & Schols, A.M. (2000). Skeletal muscle dysfunction in chronic obstructive pulmonary disease and chronic heart failure: Underlying mechanisms and therapy perspectives. American Journal of Clinical Nutrition, 71, 1033-1047.[Abstract/Free Full Text]
• Gutteridge, J.M., & Mitchell, J. (1999). Redox imbalance in the critically ill. British Medical Bulletin, 55, 49-75.[Abstract/Free Full Text]
• Ihrie, R.A., Reczek, E., Horner, J.S., Khachatrian, L., Sage, J., Jacks, T., et al. (2003). PERP is a mediator of p53-dependent apoptosis in diverse cell types. Current Biology, 13, 1985-1990.[CrossRef][Medline] [Order article via Infotrieve]
• Itoh, M., Oh-Ishi, S., Hatao, H., Leeuwenburgh, C., Selman, C., Ohno, H., et al. (2004). Effects of dietary calcium restriction and acute exercise on the antioxidant enzyme system and oxidative stress in rat diaphragm. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 287, R33-R38.
• Jaffer, S.O.L., Sunkara, S., Sabo, E., & Burstein, D.E. (2007). Immunohistochemical detection of antiapoptotic protein X-linked inhibitor of apoptosis in mammary carcinoma. Human Pathology, 38, 864-870.[CrossRef][Medline] [Order article via Infotrieve]
• Kleinberg, L., Lie, A.K., Florenes, V.A., Nesland, J.M., & Davidson, B. (2007). Expression of inhibitor-of-apoptosis protein family members in malignant mesotheliomas. Human Pathology, 38, 986-994.[CrossRef][Medline] [Order article via Infotrieve]
• Kolbeck, R.C., She, Z.W., Callahan, L.A., & Nosek, T.M. (1997). Increased superoxide production during fatigue in the perfused rat diaphragm. American Journal of Respiratory and Critical Care Medicine, 156, 140-145.[Abstract/Free Full Text]
• Laghi, F., Cattapan, S.E., Jubran, A., Parthasarathy, S., Warshawsky, P., Choi, Y.S., et al. (2003). Is weaning failure caused by low-frequency fatigue of the diaphragm? American Journal of Respiratory and Critical Care Medicine, 167, 120-127.[Abstract/Free Full Text]
• Li, X., Moody, M.R., Engel, D., Walker, S., Clubb, F.J., Jr., Sivasubramanian, N., et al. (2000). Cardiac-specific overexpression of tumor necrosis factor-alpha causes oxidative stress and contractile dysfunction in mouse diaphragm. Circulation, 102, 1690-1696.[Abstract/Free Full Text]
• Mayr, M., Hu, Y., Hainaut, H., & Xu, Q. (2002). Mechanical stress-induced DNA damage and rac-p38MAPK signal pathways mediate p53-dependent apoptosis in vascular smooth muscle cells. Journal of the Federation of American Societies for Experimental Biology, 16, 1423-1425.
• Nethery, D., Callahan, L.A., Stofan, D., Mattera, R., DiMarco, A., & Supinski, G. (2000). PLA(2) dependence of diaphragm mitochondrial formation of reactive oxygen species. Journal of Applied Physiology, 89, 72-80.[Abstract/Free Full Text]
• Nowak, M., Koster, C., & Hammerschmidt, M. (2005). PERP is required for tissue-specific cell survival during zebrafish development. Cell Death and Differentiation, 12, 52-64.[CrossRef][Medline] [Order article via Infotrieve]
• Pelech, S., Sutter, C., & Zhang, H. (2003). Kinetworks protein kinase multiblot analysis. Methods in Molecular Biology, 218, 99-111.[Medline] [Order article via Infotrieve]
• Pierce, J.D., Clancy, R.L., Smith-Blair, N., & Kraft, R. (2002). Treatment and prevention of diaphragm fatigue using low-dose dopamine. Biological Research for Nursing, 3, 140-149.[Abstract/Free Full Text]
• Pierce, J.D., Goodyear-Bruch, C., Hall, S., & Clancy, R.L. (2006). Effect of dopamine on rat diaphragm apoptosis and muscle performance. Experimental Physiology, 91, 731-740.[Abstract/Free Full Text]
• Pierce, J.D., Goodyear-Bruch, C., Jegathesan, J., & Clancy, R. (2004). Diaphragm apoptosis: Alleviation by dopamine. Critical Care Medicine, 32(Suppl), A121.
• Pierce, J.D., Jegathesan, J., Arnett, M.D., Reed, G.D., Masure, R.D., & Clancy, R.D. (2004). Dopamine alleviation of reactive oxygen species mediated diaphragm fatigue. Journal of the Federation of American Societies for Experimental Biology, 18, Abstract no. 702.702. 2004 Experimental Biology meeting abstract.
• Powers, S.K., Kavazis, A.N., & DeRuisseau, K.C. (2005). Mechanisms of disuse muscle atrophy: Role of oxidative stress. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 288, R337-344.
• Qin, Y.M., Auh, S., Blokh, L., Long, C., Gagnon, I., & Hamann, K.J. (2007). TNF-alpha induces transient resistance to Fas-induced apoptosis in eosinophilic acute myeloid leukemia cells. Cellular and Molecular Immunology, 4, 43-52.
• Reczek, E.E., Flores, E.R., Tsay, A.S., Attardi, L.D., & Jacks, T. (2003). Multiple response elements and differential p53 binding control PERP expression during apoptosis. Molecular Cancer Research, 1, 1048-1057.[Abstract/Free Full Text]
• Sakudo, A., Lee, D.C., Saeki, K., Nakamura, Y., Inoue, K., Matsumoto, Y., et al. (2003). Impairment of superoxide dismutase activation by N-terminally truncated prion protein (PrP) in PrP-deficient neuronal cell line. Biochemical and Biophysical Research Communications, 308, 660-667.[CrossRef][Medline] [Order article via Infotrieve]
• Simon, H.U., Haj-Yehia, A., & Levi-Schaffer, F. (2000). Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis, 5, 415-418.[CrossRef][Medline] [Order article via Infotrieve]
• Simpson, J.A., Van Eyk, J., & Iscoe, S. (2004). Respiratory muscle injury, fatigue and serum skeletal troponin I in rat. Journal of Physiology, 554(Part 3), 891-903.[Abstract/Free Full Text]
• Siu, P.M., Pistilli, E.E., Butler, D.C., & Alway, S.E. (2005). Aging influences cellular and molecular responses of apoptosis to skeletal muscle unloading. American Journal of Physiology: Cell Physiology, 288, C338-C349.[CrossRef]
• Smith-Blair, N.J., Pierce, J.D., & Clancy, R.L. (2003). The effect of dobutamine infusion on fractional diaphragm thickening and diaphragm blood flow during fatigue. Heart and Lung, 32, 111-120.[CrossRef]
• Stangel, M., Zettl, U.K., Mix, E., Zielasek, J., Toyka, K.V., Hartung, H.P., et al. (1996). H2O2 and nitric oxide-mediated oxidative stress induce apoptosis in rat skeletal muscle myoblasts. Journal of Neuropathology and Experimental Neurology, 55, 36-43.[Medline] [Order article via Infotrieve]
• Supinski, G., Stofan, D., Ciufo, R., & DiMarco, A. (1997). N-acetylcysteine administration alters the response to inspiratory loading in oxygen-supplemented rats. Journal of Applied Physiology, 82, 1119-1125.[Abstract/Free Full Text]
• Tews, D.S., Behrhof, W., & Schindler, S. (2005). Expression patterns of initiator and effector caspases in denervated human skeletal muscle. Muscle and Nerve, 31, 175-181.[CrossRef][Medline] [Order article via Infotrieve]
• Vassilakopoulos, T., & Petrof, B.J. (2004). Ventilator-induced diaphragmatic dysfunction. American Journal of Respiratory and Critical Care Medicine, 169, 336-341.[Free Full Text]
• Wei, Y.H., & Lee, H.C. (2002). Oxidative stress, mitochondrial DNA mutation, and impairment of antioxidant enzymes in aging. Experimental Biology and Medicine (Maywood), 227, 671-682.
• Yen, G.C., & Hsieh, C.L. (1997). Antioxidant effects of dopamine and related compounds. Bioscience, Biotechnology, and Biochemistry, 61, 1646-1649.[Medline] [Order article via Infotrieve]
• Yokoyama, I., Negita, M., Liu, D.G., Nagasaka, T., Kobayashi, T., Hayakawa, A., et al. (2002). Prevention of free-radical induced apoptosis by induction of human recombinant Cu, Zn-SOD in pig endothelial cells. Transplant International, 15, 220-225.[CrossRef][Medline] [Order article via Infotrieve]
• Yu, Y., Wang, J., Yuan, H., Qin, F., Zhang, N., Li, Y.Y., et al. (2006). Characterization of human dopamine responsive protein DRG-1 that binds to p75NTR-associated cell death executor NADE. Brain Research, 1100, 13-20.[CrossRef][Medline] [Order article via Infotrieve]
• Zhao, L.M., Xiong, S.D., Niu, R.J., Xu, Y.J., & Zhang, Z.X. (2003). Effect of Shen-Mai injection and aminophylline on diaphragmatic muscle cell apoptosis and related gene expression in rats with chronic hypoxia. Zhonghua Jie He He Hu Xi Za Zhi, 26, 606-609.[Medline] [Order article via Infotrieve]

Biological Research For Nursing, Vol. 9, No. 4, 293-300 (2008)
DOI: 10.1177/1099800408314573


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Goodyear-Bruch, C. A. Articles by Pierce, J. D. Search for Related Content PubMed PubMed Citation Articles by Goodyear-Bruch, C. A. Articles by Pierce, J. D. Social Bookmarking                         What's this?